The aim of the project is to develop mass spectrometric methodologies and protein separation techniques for the quantitative analysis of the entire proteome of a single cell. Ultimately we will be studying angiogenesis in a unique model system - consomic rat strains (chromosome substitution strains) developed by the MCW PGA project. The technological and experimental systems will be complemented by the use of bioinformatics to store, process, integrate and explore this proteomic data in conjunction with the phenotype, genotype and microarray data generated by the MCW PGA and other public resources such as the Rat Genome Database.
This project has four main components:
| Components |
Role |
| 1. Proteomics & Technology Development |
Development of improved technologies to extend the sensitivity, resolution and mass range of the analysis process. |
| 2. Animal Models & Experimental Systems |
Development of the consomic rat models that will ultimately provide model systems for the study of angiogenesis. |
| 3. Protein Analysis & Technology Implementation |
Development of protein separation techniques, implementation of new MS technologies, analysis of consomic samples. |
| 4. Bioinformatics |
Data warehousing, implementation of data analysis and data mining tools, development of novel algorithms and databases in support of proteomic studies. |
