Program to calculate accurate MWT and pI values from proteins.
Use Simple - Advanced
Data:
Data Format:
FASTA Format Sequences Accession Numbers
File upload:
pK values
Basic Amino Acids
AA
Primary
N-Terminal
C-Terminal
K
R
H
Acidic Amino Acids
D
E
C
Y
Terminal Amino Acids
Amino Acid
N-term
C-Term
G
A
S
P
V
T
I
L
N
Q
M
F
W
Protein Modifications
Terminal Ends Blocked N Terminus C Terminus Both ends
Deamidation Asparagine(N) 1 1 to 2 1 to 3 1 to 4 1 to 5 1 to 10 Glutamine(Q) 1 1 to 2 1 to 3 1 to 4 1 to 5 1 to 10
Phosphorylation Phosphoserine/threonine(S/T) 1 1 to 2 1 to 3 1 to 4 1 to 5 1 to 10 pKa1 pKa2 Phosphotyrosine(Y) 1 1 to 2 1 to 3 1 to 4 1 to 5 1 to 10 pKa1 pKa2
Additional Modifications Modification 1 Name Type Acidic Basic pKa1 pKa2 Modification 2 Name Type Acidic Basic pKa1 pKa2 Modification 3 Name Type Acidic Basic pKa1 pKa2
Display options:
Input Sequence Title Lines Amino Acid Sequence Amino Acid Composition
Output locations:
Display on Screen Save to Tab Delim Text File Plot gel image Low pH High pH pH step Low MWT (kDa) High MWT (kDa) MWT step (kDa)
Output options:
Detailed header Column headings Input Acc. Numbers Acc. Numbers Sequence description Monoisotopic mass Average isotopic mass pI
